Soil erosion by water is a serious global ecological issue that leads to land degradation and threatens ecosystem sustainability. Splash erosion resulting from raindrop impact is the first stage of the erosion process and is mainly responsible for the detachment and migration of soil surface aggregates. In humid tropical regions, however, there has been little quantitative analysis of the relations between splash erosion and variable aggregate characteristics such as stability, particle size distribution and organic matter content. The objective of the current study was to determine how rain splash erosion is related to soil aggregate characteristics under land-use change, i.e., the conversion of tropical rainforest (TR) into rubber plantation (RP). Splash cups containing dry-sieved aggregate samples with eight size classes were exposed to natural rainfall to measure the splash erosion rate. The results showed that the initial aggregate organic carbon, water-stable aggregate index (WSA) and mean weight diameter (MWD) decreased by 31%, 9% and 48%, respectively, after 32 years of rubber cultivation. These degenerations in aggregate properties increased the susceptibility of soil aggregates to erosion and mutually contributed to a higher average splash erosion rate in RP (1.20 kg m^–2) than in TR (1.09 kg m^–2), regardless of aggregate size fractions. Splash erosion rates for all aggregate sizes were significantly positively correlated with rainfall kinetic energy, rainfall amount and intensity during the study period. The average splash erosion rate of aggregates first increased and then decreased with a decline in aggregate size in both TR and RP. The minimum and maximum splash rates were observed within 10–8 mm and 0.5–0.25 mm size fractions, respectively. In particular, the proportion of small-sized aggregates (i.e., 1–0.15 mm) with a relatively high splash erosion rate increased remarkably after land-use change. This exerted a negative impact on splash erosion control as well as on the sustainable development of rubber cultivation. Increasing the additional input of organic materials (e.g., intercropping cash crops with rubber trees) may help to enhance large aggregate formation and stabilization, which could minimize the risk of splash erosion at the aggregate scale for eroded rubber plantation ecosystems.