Comparative phylogenetic analyses of Chinese Horsfieldia (Myristicaceae) using complete chloroplast genome sequences
| 论文编号: | |||||||||
| 作者: | Cai CN | ||||||||
| 刊物名称: | Journal of Systematics and Evolution | ||||||||
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| 论文题目英文: | Comparative phylogenetic analyses of Chinese Horsfieldia (Myristicaceae) using complete chloroplast genome sequences | ||||||||
| 年: | Dec 2019 | ||||||||
| 卷: | online | ||||||||
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| 联系作者: | Li J | ||||||||
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| 影响因子: | 4.04 | ||||||||
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| 摘要: | The biologist's ruler for biodiversity is the species and accurate species identification is fundamental to the conservation of endangered species and in‐depth biological scientific exploration. However, the delimitation and affinities of Horsfieldia in China has been controversial, owing in part to very low levels of molecular divergence within the family Myristicaceae. Because species boundaries and phylogenetic relationships within Horsfieldia are also unclear, 13 samples were collected across its distribution in China and their genomes were subjected to shotgun sequencing using Illumina platforms. A total of 40 487 994–84 801 416 pair‐end clean reads were obtained and after assembly, the complete chloroplast genome was recovered for all samples. Annotation analysis revealed a total of 112 genes, including 78 protein‐coding genes, 30 tRNA and four rRNA genes. Six variable loci (petN‐psbM, trnH‐psbA, ndhC‐trnV, psbJpsbL, ndhF, and rrn5‐rrn23) were identified. Phylogenetic analyses strongly support the presence of four distinct species of Horsfieldia in China. In addition, samples that had been identified previously as H. kingii were indistinguishable from those of H. prainii, suggesting that if H. kingii does occur in China, it was not collected in this study. Similarly, the chloroplast genome of one H. hainanensis sample from Guangxi province was identical to H. tetratepala, suggesting that the distribution range of H. hainanensismay be narrower than assumed previously. The phylogenetic relationships between the Chinese Horsfieldia species based on the whole chloroplast genomes was supported strongly, indicating the potential for using entire chloroplast genomes as super‐barcodes for further resolution of the phylogeny of the genus Horsfieldia. | ||||||||
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