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Arabidopsis VQ10 interacts with WRKY8 to modulate basal defense against Botrytis cinerea 

作者: Chen JQ
刊物名称: Journal of Integrative Plant Biology
论文题目英文: Arabidopsis VQ10 interacts with WRKY8 to modulate basal defense against Botrytis cinerea
年: Oct 2018
卷: 60
期: 10
页: 956-969
联系作者: Yu DQ
影响因子: 3.092
摘要: Recent studies in Arabidopsis have revealed that some VQ motif‐containing proteins physically interact with WRKY transcription factors; however, their specific biological functions are still poorly understood. In this study, we confirmed the interaction between VQ10 and WRKY8, and show that VQ10 and WRKY8 formed a complex in the plant cell nucleus. Yeast two‐hybrid analysis showed that the middle region of WRKY8 and the VQ motif of VQ10 are critical for their interaction, and that this interaction promotes the DNA‐binding activity of WRKY8. Further investigation revealed that the VQ10 protein was exclusively localized in the nucleus, and VQ10 was predominantly expressed in siliques. VQ10 expression was strongly responsive to the necrotrophic fungal pathogen, Botrytis cinerea and defense‐related hormones. Phenotypic analysis showed that disruption of VQ10 increased mutant plants susceptibility to the fungal pathogen B. cinerea, whereas constitutive‐expression of VQ10 enhanced resistance to B. cinerea. Consistent with these findings, expression of the defense‐related PLANT DEFENSIN1.2 (PDF1.2) gene was decreased in vq10 mutant plants, after B. cinereainfection, but increased in VQ10‐overexpressing transgenic plants. Taken together, our findings provide evidence that VQ10 physically interacts with WRKY8 and positively regulates plant basal resistance against the necrotrophic fungal pathogen B. cinerea.
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