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Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo 

 
论文编号:
作者: Fan QF
刊物名称: Journal of Chromatography A
所属学科:
论文题目英文: Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
年: 2018 APR
卷:
期: 2018
页:
联系作者: Song QS
收录类别:
影响因子: 3.981
参与作者:
备注:
摘要:

Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds.

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