Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
| 论文编号: | |||||||||
| 作者: | Fan QF | ||||||||
| 刊物名称: | Journal of Chromatography A | ||||||||
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| 论文题目英文: | Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo | ||||||||
| 年: | 2018 APR | ||||||||
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| 期: | 2018 | ||||||||
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| 联系作者: | Song QS | ||||||||
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| 影响因子: | 3.981 | ||||||||
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| 摘要: | Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds. | ||||||||
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