Google Search Baidu Search
(多个关键字请用"空格"格开)
您当前的位置:首页 > 科研成果 > 论文
论文

POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 (OsPRI1) positively regulates iron homeostasis in rice 

 
论文编号:
作者: Zhang HM
刊物名称: Plant Physiology
所属学科:
论文题目英文: POSITIVE REGULATOR OF IRON HOMEOSTASIS 1 (OsPRI1) positively regulates iron homeostasis in rice
年: Jul 2017
卷: online
期:
页:
联系作者: Liang G; Yu DQ
收录类别:
影响因子: 6.456
参与作者:
备注:
摘要:

OsHRZ1 is a potential iron-binding sensor. However, it is unclear how OsHRZ1 transmits signals. In this study, we reveal that OsPRI1 interacts with OsHRZ1. A loss-of-function mutation to OsPRI1 increased the sensitivity of plants to Fe-deficient conditions and downregulated the expression of Fe-deficiency-responsive genes. Yeast one-hybrid and electrophoretic mobility shift assay results suggested that OsPRI1 binds to the OsIRO2 and OsIRO3 promoters. In vitro ubiquitination experiments indicated that OsPRI1 is ubiquitinated by OsHRZ1. Cell-free degradation assays revealed that the stability of OsPRI1 decreased in wild type roots, but increased in the hrz1-2 mutant, suggesting OsHRZ1 is responsible for the instability of OsPRI1. The hrz1-2 seedlings were insensitive to Fe-deficient conditions. When the pri1-1 mutation was introduced into hrz1-2 mutants, the pri1hrz1 double mutant was more sensitive to Fe deficiency than the hrz1-2 mutant. Additionally, the expression levels of Fe-deficiency-responsive genes were lower in the hrz1pri1 double mutant than in the hrz1-2 mutant. Collectively, these results imply that OsPRI1, which is ubiquitinated by OsHRZ1, mediates rice responses to Fe-deficiency by positively regulating OsIRO2 and OsIRO3 expression as part of the OsHRZ1-OsPRI1-OsIRO2/3 signal transduction cascade.

论文下载: 下载地址
   

关闭窗口

返回首页

东南亚中心2.jpg
前往中国植物园联盟网站
W020160606696316538360.jpg
logo3.png