Google Search Baidu Search
(多个关键字请用"空格"格开)
您当前的位置:首页 > 科研成果 > 论文
论文

DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China 

 
论文编号:
作者: Liu ZF; Ci XQ
刊物名称: PLoS One
所属学科:
论文题目英文: DNA barcoding evaluation and implications for phylogenetic relationships in Lauraceae from China
年: Apr 2017
卷: 12
期: 4
页: e0175788
联系作者: Li J
收录类别:
影响因子: 3.057
参与作者:
备注:
摘要:

Lauraceae are an important component of tropical and subtropical forests and have major ecological and economic significance. Owing to lack of clear-cut morphological differences between genera and species, this family is an ideal case for testing the efficacy of DNA barcoding in the identification and discrimination of species and genera. In this study, we evaluated five widely recommended plant DNA barcode loci matK, rbcL, trnH–psbA, ITS2 and the entire ITS region for 409 individuals representing 133 species, 12 genera from China. We tested the ability of DNA barcoding to distinguish species and as an alternative tool for correcting species misidentification. We also used the rbcL+matK+trnH–psbA+ITS loci to investigate the phylogenetic relationships of the species examined. Among the gene regions and their combinations, ITS was the most efficient for identifying species (57.5%) and genera (70%). DNA barcoding also had a positive role for correcting species misidentification (10.8%). Furthermore, based on the results of the phylogenetic analyses, Chinese Lauraceae species formed three supported monophyletic clades, with the Cryptocarya group strongly supported (PP = 1.00, BS = 100%) and the clade including the Persea group, Laureae and Cinnamomum also receiving strong support (PP = 1.00, BS = 98%), whereas the CaryodaphnopsisNeocinnamomum received only moderate support (PP = 1.00 and BS = 85%). This study indicates that molecular barcoding can assist in screening difficult to identify families like Lauraceae, detecting errors of species identification, as well as helping to reconstruct phylogenetic relationships. DNA barcoding can thus help with large-scale biodiversity inventories and rare species conservation by improving accuracy, as well as reducing time and costs associated with species identification.

论文下载: 下载地址
   

关闭窗口

返回首页

东南亚中心2.jpg
前往中国植物园联盟网站
W020160606696316538360.jpg
logo3.png