The Arabidopsis WRKY transcription factors WRKY12 and WRKY13 oppositely regulate flowering under short-day conditions
| 论文编号: | |||||||||
| 作者: | Li W; Wang HP | ||||||||
| 刊物名称: | Molecular Plant | ||||||||
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| 论文题目英文: | The Arabidopsis WRKY transcription factors WRKY12 and WRKY13 oppositely regulate flowering under short-day conditions | ||||||||
| 年: | Sep 2016 | ||||||||
| 卷: | online | ||||||||
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| 联系作者: | Yu DQ | ||||||||
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| 影响因子: | 7.142 | ||||||||
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| 摘要: | In plants, photoperiod is an important cue for determining flowering. An earlier floral transition is observed in Arabidopsis thaliana under long-day (LD) than under short-day (SD) conditions, with flowering under the latter being mainly regulated by the plant hormone gibberellin (GA). Here, we report two WRKY proteins that oppositely function in controlling flowering time under short-day conditions. Phenotypic analysis showed that disruption of WRKY12 caused a delay in flowering, while disruption of WRKY13 induced flowering. Promoter-swap experiments displayed the negatively correlated expression profiles of these proteins during the regulation of flowering time. Molecular and genetic analysis demonstrated that the floral inductive signal FRUITFULL (FUL) as a direct downstream target gene of WRKY12 and WRKY13. Through yeast two-hybrid screening, the DELLA proteins GIBBERELLIN INSENSITIVE (GAI) and RGA-LIKE1 (RGL1) were identified as interacting with WRKY12 and WRKY13. The interactions between DELLAs and the WRKYs interfered with the transcriptional activity of the WRKY proteins. Moreover, WRKY12 and WRKY13 partly mediated the effect of GA3 on the control of flowering time. Taken together, our study indicates that WRKY12 and WRKY13 oppositely modulate flowering time under SD conditions. | ||||||||
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