WRKY57 Regulates JAZ Genes Transcriptionally to Compromise Botrytis cinerea Resistance in Arabidopsis
论文编号: |
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作者: |
Jiang YJ |
刊物名称: |
Plant Physiology |
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论文题目英文: |
WRKY57 Regulates JAZ Genes Transcriptionally to Compromise Botrytis cinerea Resistance in Arabidopsis |
年: |
Jun 2016 |
卷: |
online |
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页: |
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联系作者: |
Yu DQ |
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影响因子: |
6.280 |
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摘要: |
Although necrotrophic pathogens cause many devastating plant diseases, our understanding of the plant defense response to them is limited. Here, we found that loss of function of WRKY57 enhanced the resistance of Arabidopsis thaliana against Botrytis cinerea infection. Further investigation suggested that the negative regulation of WRKY57 against B. cinerea depends on the JA signaling pathway. Chromatin immunoprecipitation experiments revealed that WRKY57 directly binds to the promoters of JASMONATE ZIM-DOMAIN 1(JAZ1) and JAZ5, encoding two important repressors of the JA signaling pathway, and activates their transcription. In vivo and in vitro experiments demonstrated that WRKY57 interacts with nuclear-encoded SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2. Further experiments display that the same domain, the VQ motif, of SIB1 and SIB2 interact with WRKY3 and WRKY57. Moreover, transient transcriptional activity assays confirmed that WRKY57 and WRKY33 competitively regulate JAZ1 and JAZ5, SIB1 and SIB2 further enhance these competitions of WRKY57 to WRKY33. Therefore, coordinated regulation of Arabidopsis against B. cinerea by transcription activators and repressors would benefit plants by allowing fine regulation of defense. |
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