Two young miRNAs originating from target duplication mediate nitrogen starvation adaptation via regulation of glucosinolate synthesis in Arabidopsis thaliana
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| 作者: | He Hua | ||||||||
| 刊物名称: | Plant Physiology | ||||||||
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| 论文题目英文: | Two young miRNAs originating from target duplication mediate nitrogen starvation adaptation via regulation of glucosinolate synthesis in Arabidopsis thaliana | ||||||||
| 年: | Dec 2013 | ||||||||
| 卷: | online | ||||||||
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| 联系作者: | Yu DQ | ||||||||
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| 影响因子: | 6.555 | ||||||||
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| 摘要: | Nitrogen (N) is an essential macronutrient required for plant growth and development. A number of genes respond to N starvation conditions. However, the functions of most of these N-starvation responsive genes are unclear. Our recent survey suggested that many miRNAs are responsive to N starvation in Arabidopsis thaliana. Here, we identified a new miRNA (miR5090) from the complementary transcript of the MIR826 gene. Further investigation uncovered that both miRNA genes recently evolved from the inverse duplication of their common target gene, AOP2. Similar to miR826, miR5090 is induced by N starvation. In contrast, the AOP2 transcript level was negatively correlated with miR826 and miR5090 under N starvation. GUS-fused AOP2 expression suggested that AOP2 was post-transcriptionally suppressed by miR826 and miR5090. miRNA transgenic plants with significantly low AOP2 expression accumulated much less methionine-derived glucosinolates, phenocopying the aop2 mutants. Most glucosinolate synthesis associated genes were repressed under N starvation conditions. Furthermore, miRNA transgenic plants with less glucosinolate displayed enhanced tolerance to N starvation, such as high biomass, more lateral roots, increased chlorophyll and decreased anthocyanin. Meanwhile, N-starvation-responsive genes were upregulated in transgenic plants, implying improved N uptake activity. Our study reveals a mechanism by which A.thaliana regulates the synthesis of glucosinolates to adapt to environmental changes in N availability. | ||||||||
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